P-020 Association between male age and the frequency of sperm abnormalities

Abstract Study question Does advancing male age influence the frequency of occurrence sperm abnormalities? Summary answer Advancing was associated with increased incidence certain head and tail defects – elongated head, nuclear vacuoles, coiled tail. What is known already Evaluation reproductive potential based mainly on motility morphology. Morphological features serve as a reliable indicator in predicting fertilizing capacity sperm. Nowadays, there are various selection techniques However, has been decrease semen quality. Therefore, aim this study to examine effect aging abnormalities. design, size, duration This retrospective includes 3980 men aged between 15 72 years, mean 36 years. Semen samples were collected October 2015 December 2022 private in-vitro hospital. Men divided into three groups according their age: 1) from 25 years (n = 144), 2) 45 3447), 3) over 393). Participants/materials, setting, methods Sperm morphology evaluated Kruger’s strict criteria. Totally, 23 types abnormalities determined. Head included small, large, amorphous, elongated, round, pear-shaped, double, detached acephalic cells, small or large acrosomal areas, spermatozoa without acrosome. Midpiece thick, thin, bent, asymmetric midpiece cytoplasmic droplets. Tail short, coiled, double Acrosomal vacuoles also evaluated. Statistics: ANOVA, followed by LSD post-hoc test. Main results role chance The presented mean±SD. Among studied population percentage normozoospermic 44%, while teratozoospermic 56%. total frequencies 85.9±7.9%, 34.1±12.8% 4.7±5.1%, respectively. Only 3 (13%) analysed significantly more common advanced tail.The differed 1-2, 1-3: 11.38±10.1% vs. 13.79±11.58%, p 0.015, 14.4±11. 8%, 0.008, No difference defect observed 2 (p > 0.05).Furthermore, following group comparisons 1-3, 2-3: 4.28±4.93% 5.4±6.1%, 0.04, 6.48±7.89%, < 0.001, 5.4±6.1% р=0.001, respectively.Finally, frequently compared 1: 13.79±11.58% 11.38±10.1%, 8% Limitations, reasons for caution single-institution study. Wider implications findings showed that an several abnormalities: Further studies required clarify negative impact spermatogenesis leading morphological defects. Trial registration number NA